Fig. 2
Effects of AE2 mAb on cellular uptake and Se-supply activity of SeP in vivo. a Following the scheme shown in Supplementary Fig. 4a, C57BL/6 J mice (female, 9 weeks old) were injected twice with purified hSeP (1 mg/kg intraperitoneally) or vehicle control (PBS intraperitoneally) 12 and 2 h before sampling. After perfusion with saline, excised skeletal muscle tissues were homogenized and protein samples were analysed by western blotting with anti-hSeP Ab BD1 and anti-GPx1 Ab (n = 3, means ± s.e.m.). b, c AE2 and control rat IgG1 (20 mg/kg intraperitoneally) were administered 2 h before the first injection of hSeP. Two hours after the second injection of hSeP, muscle tissues were homogenized and protein samples were analysed by western blotting (b, n = 11–13, means ± s.e.m.). *P < 0.05, Student's t-test (a, b). Se was assayed in serum and homogenate samples of muscle and liver, as described in the Methods (c, n = 5–7, means ± s.e.m.)
