Fig. 5
The SeP-neutralizing Ab improved insulin secretion by MIN6 cells. a Insulin levels of MIN6 cells were significantly decreased by excess human SeP. MIN6 cells were incubated with 10 µg/mL hSeP for the indicated time, and then whole-cell lysates were analysed by western blotting with anti-hSeP Ab BD1, anti-GPx1 Ab, and anti-insulin Ab (n = 3, means ± s.d.). The band intensity of hSeP was only evaluated in hSeP-treated cells for 24 and 48 h. **P < 0.01, vs. time 0, Tukey-ANOVA. b Insulin secretion by MIN6 cells was significantly decreased by excess human SeP. MIN6 cells were incubated with 10 µg/mL hSeP for the indicated time, and then insulin secretion induced by high glucose levels was evaluated (n = 3, means ± s.d.). **P < 0.01, vs. time 0, Tukey-ANOVA. c–e Insulin secretion of MIN6 cells was significantly improved by AE2. MIN6 cells were incubated with 10 µg/mL hSeP in the presence of AE2 and control IgG (500 µg/mL) for 48 h, and then whole-cell lysates were analysed by western blotting (c, n = 3, means ± s.d.). The band intensity of hSeP was only evaluated in hSeP-treated cells (**P < 0.01, Student's t-test). *P < 0.05, **P < 0.01, Tukey-ANOVA. MIN6 cells treated with hSeP and AE2 for 48 h were examined immunohistochemically using anti-hSeP Ab (d) or evaluation of insulin secretion induced by high glucose levels (e, n = 3, means ± s.d.). Scale bars = 10 µm. *P < 0.05, **P < 0.01, Tukey-ANOVA
