Fig. 8
Improvement of glucose metabolism in HFHSD-fed mice by SeP-neutralizing Ab. a Time-dependent change of plasma mSeP level in mice fed a high-fat, high-sucrose diet (HFHSD) and a normal diet (ND). CBB staining was used as a control for protein loading. Graphs display the results of densitometric quantification, normalized to the major protein (albumin, indicated by black arrowhead) stained with CBB (n = 3, means ± s.e.m.). *P < 0.05, Tukey-ANOVA. b Elevation of plasma Se levels in mice fed an HFHSD. Plasma Se in mice fed an HFHSD and ND at 11 weeks was determined (n = 5, means ± s.e.m.). *P < 0.05, Student's t-test. c, d Improvement of glucose tolerance in mice fed an HFHSD treated with mSeP-neutralizing Ab. Mouse SeP-neutralizing mFHR pAb or control rabbit IgG (25 mg/kg ip) was administered 24 h before glucose-tolerance tests following the scheme shown in Supplementary Fig. 8a. Glucose (0.3 g/kg body weight) was administered intraperitoneally, and blood glucose was determined at the indicated times (c, n = 5, means ± s.e.m.). *P < 0.05, **P < 0.01, Tukey-ANOVA. Area under the curve for blood glucose levels is shown in d (n = 5, means ± s.e.m.). **P < 0.01, Student's t-test. e, f Improvement of insulin sensitivity in mice fed an HFHSD treated with mSeP-neutralizing Ab. Mouse SeP-neutralizing mFHR pAb was administered 48 h before insulin tolerance tests following the scheme shown in Supplementary Fig. 8a. Insulin (10 U/kg ip) was administered, and then blood glucose was determined at the indicated times (e, n = 5, means ± s.e.m.). The area under the curve for blood glucose levels is shown (f, n = 5, means ± s.e.m.). *P < 0.05, Student's t-test. g Improvement of insulin secretion in mice fed an HFHSD by mSeP-neutralizing Ab administration. Blood insulin levels were determined during glucose tolerance tests (n = 5, means ± s.e.m.). *P < 0.05, **P < 0.01, Tukey-ANOVA. Closed circle, control IgG; open circle, mFHR pAb (c, e, g)
