Fig. 2
Signaling upstream of ERRγ expression. a qRT-PCR analysis (n ≥ 8) of IL-6 in chondrocytes treated with IL-1β and infected with 800 MOI of control virus (Ad-C) or the indicated MOI of Ad-Epas1 or Ad-Slc3918. b, c Detection of IL-6 in chondrocytes treated with IL-1β (1 ng ml–1) by immunofluorescence microscopy (b) and Western blotting (c). d, e Western blot (d) and qRT-PCR (e) analysis of ERRγ, MMP3, and MMP13 in chondrocytes treated with IL-6 (n = 10). MMP3 and MMP13 proteins in conditioned medium of chondrocyte cultures were detected by Western blotting. f qRT-PCR analysis (n ≥ 8) of ERRγ mRNA levels in chondrocytes treated with IL-1β (left panel). qRT-PCR analysis (n ≥ 8) of ERRγ mRNA levels in chondrocytes treated with IL-6 siRNA and exposed to IL-1β (middle panel). qRT-PCR analysis (n ≥ 8) of ERRγ mRNA levels in chondrocytes treated with AG490 and exposed to IL-6 (right panel). Values are presented as means ± s.e.m. (*P < 0.05, **P < 0.005, and ***P < 0.0005. One-way ANOVA)
