Fig. 5
Failure of Polo to localize to the nucleus in prophase results in multiple mitotic defects. a In stable cell lines expressing H2A-RFP, expression of Polo-GFP (WT or mutants as indicated) was induced and cells were transfected with Polo 3′ UTR dsRNA (or control dsRNA). The next day, protein extracts were analyzed by western blots. *Non-specific band. b Cells from a were filmed. Arrowheads show lagging chromosomes. Asterisks indicate nuclei in a binucleated cell. Bar: 5 μm. c The time between prophase (visible onset of chromosome condensation, T 0) and anaphase was measured. d Quantification of cells with lagging chromosomes during division. e Quantification of cells showing a cytokinesis failure. c–e At least 40 cell divisions were scored for each condition in three independent experiments. Error bars: SD (*P < 0.05; **P < 0.01; ***P < 0.001; Student’s t test; ns non-significant)
