Fig. 2 | Nature Communications

Fig. 2

From: Mrg15 stimulates Ash1 H3K36 methyltransferase activity and facilitates Ash1 Trithorax group protein function in Drosophila

Fig. 2

Mrg15 stimulates the histone methyltransferase activity of Ash1C in vitro. a Radiography and scintillation results show that recombinant Mrg15, but not Nurf55, stimulates the activity of Ash1C. b Radiography and scintillation results show that Ash1C-Mrg15 complex has stronger enzymatic activity than Ash1C alone. c Radiography and scintillation results show Mrg15Δchromo can stimulate the activity of Flag-Ash1C-His. d Kinetic characterization of Ash1C and Ash1C-Mrg15 complex with titration of recombinant oligonucleosomes. Data are plotted as mean ± s.e.m. (n = 2). e V max and K m values of Ash1C and Ash1C-Mrg15 were determined for nucleosomes with 0.23 µM of S-[methl-3H] adenosylmethionine. Data are plotted as mean + s.e.m. (n = 2). Student's t-test was used to determine significance (*P < 0.05). NS, not significant

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