Fig. 1
Mitochondrial and histone stress modulate histone levels and trigger sHSP response. a Histone western blots from N2 worms after treatment with cco-1 or his-3 RNAi. Results shown are from three technical replicates. Similar results were obtained in two to three independent experiments. b Histone H3 western blots from N2 worms after treatment with mrps-5 RNAi or doxycycline (Dox). Results shown are from three technical replicates. Similar results were obtained in two to three independent experiments. c, d Chaperone expression in N2 worms after treatment with c cco-1 or d his-3 RNAi. In c and d, N2 worms were collected for qRT-PCR analysis at L4 stage. Bars represent mRNA levels relative to empty vector with error bars indicating mean ± s.d. of three biological replicates, each with three technical replicates (*P < 0.05, **P < 0.01; unpaired Student’s t-test). e, f sHSP expression in N2 and hsf-1(sy441) mutant worms after treatment with e cco-1 or f his-3 RNAi. In e and f, worms were collected for qRT-PCR analysis at L4 stage. Bars represent mRNA levels relative to N2 worms treated with empty vector with error bars indicating mean ± s.d. of three biological replicates, each with three technical replicates (*P < 0.05, **P < 0.01, ***P < 0.001; one-way ANOVA with Tukey’s post test)
