Fig. 3
F351A suppresses the IO state and eliminates hook currents. a Schemes to illustrate the location of the mutation in the KCNQ1 subunit (left) and show that the IO state was suppressed by F351A mutation (right). b Representative hook currents of F351A recorded in 100 mM K+ solution. The pre-pulses were +40 mV, with 0.02–4 s time durations, and the test pulse was 2 s long at −120 mV. The right panel shows tail currents with an expanded time scale. c VCF recordings of F351A currents (black) and florescence (blue). Right panel shows the G–V (open circle) and F–V (solid circle) relationships of F351A with the F 1 and F 2 components (dotted blue lines). The red dotted line is the G–V relationship of IKs channels. n ≥ 4. Error bars are the SEM
