Fig. 4

Enhancement of spatial pattern separation increases the dNGIs survival. a The experimental schedules (top) show that mice at 120 ± 2 days old of age were applied with TBS. 7 days after the beginning of TBS, mice were performed with 16-days PAL. The percentage (bottom) of corrections in 60-days PAL is plotted (mean ± SEM, n = 11 mice/group). b The experimental schedules (top) show that mice (after the completion of PAL) were administered with a single dose of BrdU. 7 days after the administration, mice were trained with 2-CSD. 4 days after the beginning of the training, mice were treated with 16-days TBS. Following the completion of 20-days training, mice were applied for a 5-day block test on each separation (high or low). Immediately after end of the tests, mice were euthanized for BrdU staining. The numbers (bottom) of trials that are required to reach a criterion at a high or a low separation of 2-CSD are plotted (mean ± SEM, n = 11 mice/group, F _1.43 = 11.02, *p = 0.00082, two-way ANOVA). c The numbers of trials that are required to reach a criterion at a low separation in b are plotted against the number of BrdU⁺ cells from the individual mice