Fig. 5

Inhibition of spatial pattern separation reduces the dNGIs survival. a The experimental schedules (top) show that mice at 120 ± 2 days old of age were applied with ALL. 7 days after the beginning of ALL, mice were performed with 16-days PAL. The percentage (bottom) of corrections in 60-days PAL is plotted (mean ± SEM, n = 11 mice/group). b The experimental schedules (top) show that mice (after the completion of PAL) were administered with a single dose of BrdU. 7 days after the administration, mice were trained with 2-CSD. 4 days after the beginning of the training, mice were treated with 16-days ALL. The mice were applied for a 5-day block test on each separation (high or low). Immediately after end of the tests, mice were euthanized for BrdU staining. The numbers (bottom) of trials that are required to reach a criterion at a high or a low separation of 2-CSD (mean ± SEM, n = 11 mice/group, F _1.43 = 9.87; *p = 0.00098, two-way ANOVA). c, The numbers of trials that are required to reach a criterion at a low separation in b are plotted against the number of BrdU⁺ cells from the individual mice (Rsqr = 0.92 in vChATs^NpHR−/ALL vs. 0.94 in vChATs^NpHR+/ALL mice). d–f The experimental schedules d, and the actual (blue circles) and averaged trials that are required to reach a criterion at a low e, or a high f, separation of 2-CSD from the individual mice (mean ± SEM, n = 11 mice/group, F _1.43 = 9.79, *p = 0.0012, **p = 0.00082, two-way ANOVA). g The numbers of trials that are required to reach a criterion at a low separation in e are plotted against the number of BrdU⁺ cells from the individual animals that were treated with TBS (Rsqr = 0.936 in dNGIs^sM1siRNA+ vs. 0.648 in dNGIs^M1siRNA+ mice)