Fig. 1
Characterization of Vps34D761A/+ mice. a Expression of the Vps34 protein in WT and Vps34D761A/+ mouse tissues. 100 µg of protein was loaded per lane. Representative data from three independent experiments. b Lipid kinase activity associated with Vps34 in WT and Vps34D761A/+ mouse tissues. Tissue and cell homogenates were immunoprecipitated with an anti-Vps34 antibody, and an in vitro lipid kinase assay was performed using PI as substrate. Data represent mean ± SEM (non-parametric Mann–Whitney t-test) *p < 0.05, **p ≤ 0.01, ***p ≤ 0.001. 4 mice/genotype. c Expression levels of Vps34 and its binding partners in liver and brain tissues of 12-week-old mice. Tissue/cell lysates were immunoblotted using the indicated antibodies. 60 µg of protein was loaded per lane. Composite images are derived from experiments whereby equal amounts of the same cell/tissue extract were loaded on separate gels and developed with the same indicated antibodies. This was done to quantify proteins with a similar molecular weight
