Fig. 10
Impact of pharmacological inhibition of Vps34 on insulin resistance in 60% HFD-fed mice. a GTT after intraperitoneal injection of 2 g/kg of glucose after overnight starvation in 60% HFD-fed mice treated with compound 19 for 4 weeks. Data represent mean ± SEM (non-parametric Mann–Whitney t-test). 10–12 mice/genotype. *p < 0.05, **p < 0.01, ***p < 0.001. b ITT after intraperitoneal injection of 0.75 U/kg of insulin after overnight starvation in 60% HFD-fed mice treated with compound 19 for 5 weeks. Area under the curve (AUC) is shown adjacent to the graph. Data represent mean ± SEM (non-parametric Mann–Whitney t-test). 10–12 mice/genotype. p < 0.05, **p < 0.01, ***p < 0.001. c Tissue homogenates from liver isolated from overnight-starved mice were immunoblotted using the indicated antibodies. Each lane represents an individual mouse. 60 µg of protein was loaded per lane. d Schematic representation of the metabolic impact of partial systemic Vps34 inactivation, as described in the manuscript. See text for details
