Fig. 3
Impact of Vps34 inactivation on glucose tolerance, insulin sensitivity in HFD-fed mice. a Serum glucose levels in randomly fed and fasted mice. Data represent mean ± SEM (non-parametric Mann–Whitney t-test). b Serum insulin levels in randomly fed and fasted mice. Data represent mean ± SEM (non-parametric Mann–Whitney t-test). c GTT after intraperitoneal injection of 2 g/kg of glucose in mice in overnight- starved mice. Data represent mean ± SEM (non-parametric Mann–Whitney t-test). d ITT after intraperitoneal injection of 0.75 U/kg of insulin in overnight-starved mice. Data represent mean ± SEM (non-parametric Mann–Whitney t-test). e Hematoxylin and eosin (H&E) staining and Oil Red O staining of liver sections of mice after 16 weeks of HFD. Representative image derived from 6 livers/genotype of mice. Scale bar, 10 µm. f Quantification of vacuolation of 6 livers/genotype of mice after 16 weeks of HFD. a.u., arbitrary units. Data represent mean ± SEM (non-parametric Mann–Whitney t-test). g Comparison of liver weight of mice fed a NCD or HFD. Data represent mean ± SEM (non-parametric Mann–Whitney t-test). Quantification of 5–9 livers/genotype. h Triglyceride levels in liver of mice challenged with 45% HFD for 16 weeks. Data represent mean ± SEM (non-parametric Mann–Whitney t-test). i Plasma triglyceride levels of mice challenged with 45% HFD for 16 weeks. Data represent mean ± SEM (non-parametric Mann–Whitney t-test). For all experiments shown, ≥10 mice/genotype were used. *p < 0.05, **p ≤ 0.01, ***p ≤ 0.001
