Fig. 5
Mouse and human β-catenin-accumulating clusters share a common signature of senescence and SASP. a–d Gene set enrichment analysis (GSEA) (a, c) revealing that clusters from both mouse models, the Hesx1Cre/+;Ctnnb1lox(ex3)/+ and Sox2CrERT2e/+;Ctnnb1lox(ex3)/+;R26YFP/+, show a molecular signature of oncogene-induced senescence (OIS) and senescence-associated secretory phenotype (SASP). qRT-PCR analysis showing the upregulation of several senescence and SASP factors in both mouse models (b, d). Bars in b and d represent the mean, and error bars represent standard error of the mean (SEM) of three biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001, Student’s t test. e Several cytokines are upregulated in the Hesx1Cre/+;Ctnnb1lox(ex3)/+ mutant relative to the Hesx1+/+;Ctnnb1lox(ex3)/+ control pituitaries as measured in a mouse cytokine antibody array. f Gene set enrichment analysis (GSEA) demonstrating that laser-capture micro-dissected human clusters show a molecular signature of oncogene-induced senescence (OIS) and senescence-associated secretory phenotype (SASP). g Hierarchical clustering analysis of RNA-data sets from cluster and non-cluster cell populations indicating coherent grouping between specific cell compartments. h, i GSEA of the top 100 or 500 most upregulated genes in human clusters and the whole transcriptome of mouse clusters from both models demonstrating a common molecular signature
