Fig. 3
From: Oncogenic PIK3CA induces centrosome amplification and tolerance to genome doubling
p110αH1047R expression in MEFs leads to centrosome overduplication. a Representative immunofluorescence images showing extra centrosomes in p110αH1047R MEF cells composed of two centrioles. b Representative immunofluorescence images showing extra centrosomes contributing to the mitotic spindle in p110αH1047R MEF cells. c Centrosome number during the first cell cycle upon induction of p110αH1047R expression (top panel; 200 cells per genotype and time point were scored for the centrosome analysis; values = mean ± SD) and parallel analysis of DNA synthesis (assessed by BrdU incorporation; bottom panel: 10,000 cells were acquired from two independent WT and two independent p110αH1047R MEF lines. Statistically significant differences are indicated by *(P < 0.05), as determined by non-parametric Mann–Whitney t test (one-tailed). MEFs were plated and cultured overnight in serum-containing medium. The next morning, cells were serum-starved in the presence of 4-OHT for 48 h, followed by re-addition of 10% fetal bovine serum and sampling of cells at the indicated time points. d Percentage of binucleated MEFs in cultures treated 2 days with 4-OHT. Binucleation was assessed by IF using DAPI to stain DNA. A total of 200 cells were scored per genotype in 3 Pik3ca WT;Flpe-ER T2 and 3 Pik3ca H1047R+neo;Flpe-ER T2 independent MEF lines (values = mean ± SD)
