Fig. 5
From: Oncogenic PIK3CA induces centrosome amplification and tolerance to genome doubling
Efficient clustering of extra centrosomes and lack of chromosomes segregation errors early upon p110αH1047R expression. a Quantification of the number of cells with centrosome amplification that either make it through mitosis or experience mitotic slippage or cytokinesis failure. In total, 152 WT cells (50, 48 and 54 cells from 3 independent WT MEFs) and 161 Pik3ca H1047R cells (50, 61 and 50 cells from 3 independent Pik3ca H1047R MEFs) with centrosome amplification entering mitosis were analysed; values = mean ± SD). b Frequency of segregation errors occurring in anaphase of Pik3ca WT;Flpe-ER T2 and Pik3ca H1047R+neo;Flpe-ER T2 MEFs, 3 days after treatment with 4-OHT. A total of 103 WT cells from 3 independent WT MEFs (33, 32 and 38); and 101 Pik3ca H1047R cells (38, 39 and 24 from 3 independent Pik3ca H1047R MEFs) in anaphase were analyzed for the presence of chromosome segregation errors. Individual values are plotted. c Representative immunofluorescence images of mitotic spindle conformations observed in Pik3ca WT;Flpe-ER T2 and Pik3ca H1047R+neo;Flpe-ER T2 MEFs, 3 days after treatment with 4-OHT and quantification of the incidence of pseudo-bipolar and multipolar spindles in metaphases with > 2 centrosomes. 74 WT cells (29, 30 and 15 cells from three independent WT MEFs) and 83 Pik3ca H1047R cells (30, 27 and 26 cells from 3 independent Pik3ca H1047R MEFs) with centrosome amplification in prometaphase/metaphase were analyzed (values = mean ± SD). Statistically significant differences are indicated by *(P < 0.05), as determined by non-parametric Mann–Whitney t test (one-tailed)
