Fig. 6

p110α^H1047R expression provides cellular tolerance to genome doubling. a Frequency of aneuploid cells (i.e. with a number of chromosomes that differs from 40), assessed by metaphase chromosome spreads, in primary MEFs 5 days after treatment with 4-OHT. Statistically significant differences are indicated by *(P < 0.05), as determined by non-parametric Mann–Whitney t test (two-tailed). Five independent Pik3ca ^WT;Flpe-ER ^T2 and 5 independent Pik3ca ^H1047R+neo;Flpe-ER ^T2 MEFs were analyzed, and ~50 metaphase spreads counted per MEF line. b Frequency of tetraploid cells in Pik3ca ^WT;Flpe-ER ^T2 and Pik3ca ^H1047R+neo;Flpe-ER ^T2 MEFs, 3 and 5 days after treatment with 4-OHT. Cells were considered to be tetraploid (or tetraploid-derived) if the number of chromosomes per cell was ≥ 70. Three independent Pik3ca ^WT;Flpe-ER ^T2 and 3 independent Pik3ca ^H1047R+neo;Flpe-ER ^T2 MEFs were analyzed, and ~50 metaphase spreads counted per MEF line and per time point (values = mean ± SD). Statistically significant differences are indicated by *(P < 0.05), as determined by the non-parametric Mann–Whitney t test. c Analysis of chromosome numbers in mouse keratinocytes 2 days after addition of 4-OHT. One Pik3ca ^WT;Flpe-ER ^T2 and one Pik3ca ^H1047R+neo;Flpe-ER ^T2 keratinocyte culture were analyzed. d Frequency of binucleated MEFs dividing within a period of 30 h after DCB-induced tetraploidization, assessed 3 days after treatment with 4-OHT. Three independent Pik3ca ^WT;Flpe-ER ^T2 and three independent Pik3ca ^H1047R+neo;Flpe-ER ^T2 MEFs were analyzed, and 60–80 binucleated cells were tracked per MEF line (values = mean ± SD). Statistically significant differences are indicated by *(P < 0.05), as determined by the non-parametric Mann–Whitney t test (one-tailed)