Fig. 8

Impact of pharmacological blockade of PI3K pathway components on tetraploidization in primary and tumour-derived MEFs. a Impact of inhibition of p110α (by 3 µM A66) or ROCK (by 10 µM Y27632) on p110α^H1047R-induced chromosomal abnormalities in primary MEFs. One Pik3ca ^H1047R+neo;Flpe-ER ^T2 MEF line was analyzed and at least 50 metaphase spreads were counted per condition. Inhibitors were added together with the 4-OHT to induce p110α^H1047R expression. b Impact of 14 day inhibition of p110α (by 3 µM A66), Akt (by 1 µM MK-2206) or ROCK (by 10 µM Y27632) on chromosome numbers of two independent stably transformed p110α^H1047R MEF clones with a different degree of tetraploidy. c Impact of inhibition of Akt (by 1 µM Akti X) or ROCK (by 10 µM Y27632) for 21 days on chromosome numbers of p110α^H1047R Nutu clone 1 (shRNA p53-immortalized p110α^H1047R MEF clone passed through nude mice as tumour). Chromosome counting was performed on DAPI-stained metaphase spreads. Fifty spreads were counted per line and condition, unless otherwise stated