Fig. 8

Inhibition of intracellular vesicle fusion and transport by bafilomycin A1. A549 cells were treated with bafilomycin A1 (100 nM, 30 min) prior to the introduction of 4T-HSA a–d, 1P3T-HSA e–h, and Me1P3T-HSA i–l at 15 µM. Images were taken after 24 h incubation at 37 °C, 5% CO₂ with the respective complex. Mitochondria of the cells were stained with MitoTracker Deep Red represented in purple color. The corresponding experiments without bafilomycin A1 are presented in Fig. 6. Scale bar = 20 µm. Bafilomycin A1 pretreatment of cells trapped 1P3T e and Me1P3T i in their molecular states (green) within their respective compartments whereas it has no effect upon 4T a assembly (green and red indicating co-existence of molecular and assembled forms). l Co-localization studies suggest that only Me1P3T targets the mitochondria and localizes there in its molecular form (white color of the co-localization of molecular Me1P3T in green and MitoTracker in purple)