Fig. 10 | Nature Communications

Fig. 10

From: Characterization of a membrane-bound C-glucosyltransferase responsible for carminic acid biosynthesis in Dactylopius coccus Costa

Fig. 10

Activities of different DcUGT2-Strep forms synthesized in yeast. Yeast microsomes containing pYES-DEST52, DcUGT2-Strep, DcUGT2ΔMD-Strep and ΔSP-DcUGT2ΔMD-Strep were incubated with UDP-glucose and a Kermes vermilio metabolite fraction, containing kermesic acid and flavokermesic acid. Assay products were analyzed by LC-ESI(−)-MS and ion chromatograms of m/z 475.0882 (purple) and 491.0831 (blue) were extracted, corresponding to dcII and carminic acid (CA), respectively

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