Fig. 4 | Nature Communications

Fig. 4

From: Characterization of a membrane-bound C-glucosyltransferase responsible for carminic acid biosynthesis in Dactylopius coccus Costa

Fig. 4

Identification of a DcUGT glucosylating FK in vitro. Microsomes of D. coccus and of yeast expressing Strep-tagged DcUGT2, DcUGT5 and pYES-DEST52 vector, respectively, were incubated with [14C]UDP-glucose and with/without flavokermesic acid. As a control, D. coccus microsomes were incubated with the asperthecin aglucone and [14C]UDP-glucose. Formed products were separated by TLC. a Aglucone substrates tested in the in vitro glucosylation assay. b TLC-separated [14C] products formed in vitro and viewed by phosphorimaging. FK flavokermesic acid, CA carminic acid

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