Fig. 5 | Nature Communications

Fig. 5

From: Characterization of a membrane-bound C-glucosyltransferase responsible for carminic acid biosynthesis in Dactylopius coccus Costa

Fig. 5

DcUGT2 catalyzes CA and dcII formation in vitro. Yeast microsomes containing (b) pYES-DEST52 vector or (c) DcUGT2-Strep were incubated with UDP-glucose and a Kermes vermilio metabolite fraction, containing flavokermesic acid (FK) and kermesic acid (KA) (shown in a). Assay products were analyzed by HPLC-HRMS/MS. Peaks indicated in the MS base peak chromatograms (a–c) were identified by comparing retention times and mass fragmentation spectra. Mass spectra of FK, KA, dcII, and carminic acid (CA) identified in assays (d, shown in purple) stacked with those of authenticated standards (d, shown in black)

Back to article page