Fig. 4
From: NDP52 activates nuclear myosin VI to enhance RNA polymerase II transcription
NDP52 is a nuclear myosin VI binding partner. a Cartoon and structural model depicting NDP52 domains as mentioned in the text. b Immunofluorescence staining against NDP52 (green) and DNA (cyan) in HeLa cells and isolated nuclei, as performed in Fig. 1b. Arrows highlight filamentous structures. Scale bar 10 μm for whole images and 1 μm for inserts. c Western blot against NDP52 following HeLa cell fractionation. Tubulin and lamin B were used as cytoplasmic and nuclear markers, respectively. d FRET titration of MVITAIL constructs against NDP52 (1 μM). Data fitting was performed as described in Methods giving a K d as plotted in e (error bars represent SEM from three independent experiments). f Western blot against NDP52 following isolation from nuclear extracts by MVITAIL constructs. Loading controls for the recombinant proteins is shown in Supplementary Fig. 9. g Immunofluorescence staining against NDP52 (green), MVI (magenta) and DNA (cyan), as performed in Fig. 1b. Insert depicts co-localising foci. Scale bar 10 μm for whole images and 1 μm for inserts. Pearson’s coefficient is shown for HeLa and nuclei (Supplementary Fig. 5b). Error bars represent SEM from 10 images
