Table 1 Kinetic properties of MtbPYK

From: Allosteric pyruvate kinase-based “logic gate” synergistically senses energy and sugar levels in Mycobacterium tuberculosis

Ligand

Kinetic parameter

Modulators

  

None

+AMPa

+G6Pa

+AMP/G6Pa

+ATPa

+ATP/AMP/G6Pa

PEP

S 0.5 (mM)

0.41 ± 0.01

0.25 ± 0.01

0.18 ± 0.01

0.18 ± 0.01

2.20 ± 0.08

0.37 ± 0.01

 

h

1.82 ± 0.05

1.21 ± 0.04

1.24 ± 0.07

1.23 ± 0.03

2.63 ± 0.21

1.15 ± 0.03

 

k cat (s−1)

183 ± 1.2

194.8 ± 2.1

172.7 ± 2.8

188.8 ± 1.4

101 ± 2.7

136 ± 1.3

 

k cat/S 0.5

446

780

960

1049

46

367

ADP

K m (mM)

0.47 ± 0.02

N.A.

  

None

 + AMP

 + G6P

 
   

12.5 µM

25 µM

25 µM

50 µM

 

AMP

Ka0.5 (µM)

63.5 ± 2.9

N.A.

N.A.

35.7 ± 0.9

20.3 ± 1.4

 

G6P

Ka0.5 (µM)

147 ± 5.8

55.4 ± 3.1

42.2 ± 3.4

N.A.

N.A.

 
  1. k cat/S 0.5 values in s−1 mM−1
  2. h Hill coefficient, N.A. not applicable
  3. aThe concentrations of modulators in the kinetic study of PEP are: AMP—1 mM, G6P—1 mM, ATP—2 mM
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