Fig. 1
PEPD is essential for cell survival in vitro and in vivo. a Measurement of UM-UC-3 cell viability and IB analysis of PEPD after siRNA treatment. b Measurement of UM-UC-3 cell viability and IB analysis of PEPD after siRNA treatment for 24 h and then treatment with or without PEPD or PEPDG278D (His-tagged) for 48 h. c IB analysis of various proteins in UM-UC-3 cells after siRNA treatment. d UM-UC-3 cell cycle analysis by flow cytometry after siRNA treatment for 72 h. e, f UM-UC-3 tumor growth in athymic nude mice after intratumoral injection of control siRNA (open square) or PEPD siRNA (filled square) at the indicated times (arrows) and final tumor weight. g IB analysis of various proteins in tumors from 3 mice/group obtained on day 14. GAPDH and VDAC were measured to ensure the purity of subcellular fractions or as loading controls. Cells were cultured in 6-well plates (5 × 104 cells/well) for 24 h before experimental treatment in a–d. Data are means ± s.d. (n = 3) in a, b, and d (two-way ANOVA followed by Tukey multiple comparisons test) and means ± s.e.m. (n = 8) in e and f (two-tailed t-test)
