Fig. 3 | Nature Communications

Fig. 3

From: PEPD is a pivotal regulator of p53 tumor suppressor

Fig. 3

PEPD inhibits transcription-independent p53 activity. a IB analysis of p53 relocation in cells after siRNA treatment for 72 h. Lamin A, α-tubulin and VDAC were measured to ensure the purity of the subcellular fractions or as loading controls. b IB analysis of p53 binding to CYPD in mitochondria after cell treatment with siRNA for 72 h. c IB analysis of Bcl-xL binding to p53, Bax and Bak in mitochondria after cell treatment with siRNA for 72 h. d Flow cytometry analysis of MMP after cell treatment with siRNA for 72 h. Cells were cultured in 6-well plates (5–10 × 104 cells/well) for 24 h before experimental treatment. Data are means ± s.d. (n = 3)

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