Fig. 6

PRD mutation inactivates p53. a IB analysis of p53^WT and p53^mPRD. b IB analysis of various proteins in whole lysates and subcellular fractions of cells treated with control siRNA or PEPD siRNA for 48 h. c, d IB analysis of p53^WT and p53^mPRD, as well as analysis of cell viability at 48 h after plasmid transfection. Cells were cultured in 24-well plates (5 × 10³ cells/well) for 24 h before plasmid transfection. e, f Analysis of cell viability and IB analysis of various proteins in cells treated with siRNA for 48 h and then transfected with the plasmid for 48 h. GAPDH, α-tubulin and VDAC were measured to ensure the purity of the subcellular fractions or as loading controls. Cells were cultured in 6-well plates (8 × 10³ cells/well) for 24 h before experimental treatment. Data are means ± s.d. (n = 3); one-way ANOVA followed by Tukey multiple comparisons test in d; two-way ANOVA followed by Tukey multiple comparisons test in e; n.s., not significant