Fig. 3
From: Parallel derivation of isogenic human primed and naive induced pluripotent stem cells
T2iLGö hiNPSCs metabolic profile is closely related to preimplantation epiblast. a Genes coding proteins of the electron transport chain, located in the inner membrane of the mitochondria, are upregulated in human epiblast cells and T2iLGö hiNPSCs in comparison to their primed counterparts. Relative expression of genes related to oxidative phosphorylation pathway for hESC, morula and epiblast samples analyzed by single-cell RNA-seq (left), and analyzed by DGE-seq for primed or naive hPSCs (right). Genes were classified by mitochondrion complex and hierarchically clustered. b T2iLGö hiNPSCs have higher metabolic activity than their isogenic counterparts in RSeT and KSR+FGF2. A SeaHorse apparatus was used to measure the oxygen consumption rate and the extracellular acidification rate of hiNPSC and hiPSC lines, maintained in indicated culture conditions. This figure presents six biological replicates. Each symbol in the panel is the average of a technical triplicate. c T2iLGö hiNPSCs have a higher resistance to inhibition of glycolysis. Quantification of colony numbers obtained after culture with the indicated concentrations of 2-deoxy-D-glucose. Primed cells were seeded in StemMACS™ iPS Brew XF, and naive cells were seeded in the indicated medium. Error bars indicate s.d. of three technical replicates. The presented experiment is representative of four independent experiments
