Fig. 5
From: Parallel derivation of isogenic human primed and naive induced pluripotent stem cells
T2iLGö hiNPSCs have a X-chromosome status related to preimplantation epiblast. a T2iLGö hiNPSCs or high-passage KSR-FGF2 do not display H3K27me3 foci. Indicated cell lines were analyzed by immunofluorescence for H3K27me3. This experiment is representative of three technical replicates performed at different passages of the cell lines. Scale bar = 50 µm. b, c T2iLGö hiNPSCs show signs of X-chromosome reactivation. mRNA FISH analysis for b ATRX and XACT or c XIST and XACT. For each cell line represented, more than 100 cells were investigated for their nuclear expression of the indicated mRNA. Quantifications for each combination are indicated below pictures. For each table, samples distribution between FISH counting were found statistically different (p < 0.01) by a homogeneity χ2 test. Scale bar = 20 µm
