Fig. 5

CSB evicts histones from the chromatin surrounding I-PpoI-induced DSBs in vivo. a Relative occupancy of histone H2A in ddI-PpoI-expressing hTERT-RPE parental cells. Cells were either untreated (untx) or treated with Shield-1 and 4-OHT and then collected at indicated times. The x-axis represents the distance in kb upstream and downstream from the I-PpoI-induced DSB on chromosome 1, which was set as 0. The y-axis represents the relative occupancy of H2A of treated cells relative to untreated cells. Standard error of the mean (SEM) from three independent experiments are indicated. b Relative occupancy of histone H2B in ddI-PpoI-expressing hTERT-RPE parental cells. Both x- and y-axes are as described in a. SEM from three independent experiments are indicated. c Relative occupancy of histone H2A in ddI-PpoI-expressing hTERT-RPE CSB KO cells. Both x- and y-axes are as described in a. SEM from three independent experiments are indicated. d Relative occupancy of histone H2B in ddI-PpoI-expressing hTERT-RPE CSB KO cells. Both x- and y-axes are as described in a. SEM from three independent experiments are indicated. e Relative occupancy of histone H2A in ddI-PpoI-expressing hTERT-RPE CSB KO cells complemented with the vector alone, Myc-tagged wild-type CSB or Myc-tagged mutant CSB-W851R. Both x- and y-axes are as described in 5a. SEM from three independent experiments are indicated. f Relative occupancy of histone H2B in ddI-PpoI-expressing hTERT-RPE CSB KO cells complemented with the vector alone, Myc-tagged wild-type CSB or Myc-tagged mutant CSB-W851R. Both x- and y-axes are as described in a. SEM from three independent experiments are indicated