Fig. 9

CSB phosphorylations on S10 and S158 modulate the interaction of the N-terminus with the ATPase domain. a Immunofluorescence of U2OS-265 cells transfected with Myc-CSB-N in conjunction with either mCherry-LacR-CSB-ATPase or mCherry-LacR-CSB-C. Scale bars, 5 μm. b Quantification of the percentage of U2OS-265 cells from a with anti-Myc staining at FokI-induced DSBs. A total of 250 Myc-expressing cells were scored for each independent experiment in a blind manner. SDs from three independent experiments are indicated. c Quantification of the percentage of U2OS-265 cells with anti-Myc staining at FokI-induced DSBs. U2OS-265 were co-transfected with various alleles as indicated. A total of 250 Myc-expressing cells were scored for each independent experiment in a blind manner. SDs from three independent experiments are indicated. *P < 0.05 (Student t test). d Model for control of CSB chromatin remodeling activity by ATM and CDK2 in DNA DSB repair pathway choice in S/G2. See the text for details