Fig. 1
From: Identification of regulatory targets for the bacterial Nus factor complex
Transcription termination within suhB is dependent on Rho and Nus factors. RNAP (β) enrichment at suhB 5′ and 3′ regions was measured using ChIP-qPCR in wild-type MG1655, boxA(C4T/T6C), ΔnusB, nusE(A12E) or rho(R66S) mutant strains. Values are normalized to signal at the 5′ end of suhB. x-axis labels indicate qPCR amplicon position relative to suhB. Error bars represent ±1 standard deviation from the mean (n = 3). A schematic depicting the suhB gene, the transcription start site (bent arrow) and boxA (grey rectangle) is shown below the graph. Horizontal black lines indicate the position of PCR amplicons
