Fig. 2
From: Identification of regulatory targets for the bacterial Nus factor complex
A functional BoxA in the 5′ UTR of suhB. a Sequence conservation of the 100 bp upstream of suhB and its homologues across 20 Enterobacteriaceae species. The transcription start site is indicated by a bent arrow, and the BoxA and S-D sequences are indicated. b List of boxA mutations that are associated with increased suhB expression. All single nucleotide changes are indicated by an arrow. Single underline indicates a mutation that was isolated in the absence of mutations anywhere else in the cloned region; other mutants included additional mutations outside the boxA. Double underline indicates that the boxA mutation was isolated in two or more independent clones. Critical position ‘−4’ is indicated (see Supplementary Table 2). c SuhB association with the 5′ end of suhB in wild-type (‘wt’) and boxA mutant (‘boxA C4T/T6C’) strains. SuhB-FLAG occupancy was measured by ChIP-qPCR using α-FLAG antibody. Error bars represent ±1 standard deviation from the mean (n = 3)
