Fig. 4

Levels of relevant cytokines in the brain, lung, and spleen of C. neoformans-infected WT and Gal-3 KO mice. WT (blue bars) and Gal-3 KO (red bars) mice were intratracheally infected with a 50 μl suspension containing 1×10⁶ yeast cells. Organ samples were weighed and homogenized. The levels of the following cytokines in the brains (a–g), lungs (h–n), and spleens (o–u) (3, 7, and 14 days post-infection) of infected mice were determined by ELISA: IFN-γ, IL-12p40, IL-6, TNF-α, IL-10, IL-17, and IL-23. The results represent the mean ± SD of five mice per group, from a representative experiment of three assays. *p < 0.05, Student’s t-test, Gal-3 KO mice compared with WT mice