Fig. 2

Genomic localization of the VANC21 protein. a A genome-wide view showing the enrichment of FLAG-VANC21 signal. Each dot represents signal in a 10 kb region. Red dots indicate the regions with VANDAL21 copies more than 1 kb long. b Genome browser views showing the FLAG-VANC21 signals with normalized coverages (per million mapped reads) and DNA methylation profiles (0–100%) of WT and ∆AB transgenic plants at VANDAL21 copies. Each point represents proportion of methylated cytosine counted within five successive cytosine residues. VANC21 exons are colored red. Arrowheads indicate probe sites used in EMSA (Fig. 3). c–e Three contexts of DNA methylation level around VANC21 biding loci. Around the summits of VANC21-binding loci within VANDAL21 (N = 89), 500 bp-binned averages of DNA methylation profiles were plotted for WT and ∆AB transgenic plants with steps of 50 bp