Fig. 9

Exacerbation of WAT browning by a miR-327 inhibitor. a Histological analysis of adipocyte morphology (H&E), adipocytes (PERI), mitochondria (COX4) and uncoupling protein 1 (UCP1) in Inh-miR-NC- and Inh-miR-327-treated 2-week 30 °C and 2-week 4 °C-exposed scWAT. Double-headed arrows mark adipocyte diameter. Arrows point to respective positive signals. b–d Quantifications of adipocyte size and positive signals of COX4 and UCP1 in Inh-miR-NC- and Inh-miR-327-treated 2-week 30 °C and 2-week 4 °C-exposed scWAT (>30 adipocytes per field; n = 10 random fields; n = 4 mice per group). e–h Western immunoblot analysis and quantification of FGF10 and UCP1 in Inh-miR-NC- and Inh-miR-327-treated 2-week 30 °C and 2-week 4 °C-exposed scWAT. FGF10 and UCP1 protein levels were quantified as densitometric signals and normalized to β-actin (n = 4 samples per group). i scWAT weight of C57BL/6 mice receiving Inh-miR-NC or Inh-miR-327 treatment under 2-week 30 °C or 4 °C exposure (n = 6 mice per group). j, k Oxygen consumption of Inh-miR-NC- and Inh-miR-327-treated C57BL/6 mice after 2-week 30 °C or 4 °C exposure (n = 6 mice per group). l Norepinephrine-stimulated thermogenesis of Inh-miR-NC- and Inh-miR-327-treated C57BL/6 mice after 2-week 30 °C and 2-week 4 °C exposure. Oxygen consumption is presented as area under curve (AUC) (n = 4 mice per group). Scale bars, 100 μm. kDa, kilodalton. NS, not significant. *P < 0.05, **P < 0.01, and ***P < 0.001 by Student’s t-test. Data presented as mean ± s.e.m.