Fig. 3
Analyses of functional SNPs within OsUGT707A2 and OsUGT706D1. A representation of the pairwise r 2 value (a measure of linkage disequilibrium) among polymorphic sites in OsUGT707A2 (a) and OsUGT706D1 (f), where the darkness of the box corresponds to the r 2 value according to the legend. Stars represent the significant sites identified in UGT707A2 (P < 8 × 10–33) and UGT706D1 (P < 2 × 10–19), and solid stars indicate the functional SNPs. The P value is calculated using the Student’s t tests. b Correlation between relative contents of apigenin 5-O-glucose and transcript levels of OsUGT707A2 in 39 rice varieties. c Box plot for the content of apigenin 5-O-glucoside; plotted as an associated site at Chromosome 7. sf0719059405. ***P < 0.001, Student’s t tests. d Alignment of partial nucleotide sequences of OsUGT707A2 from different rice varieties. C153, C196 and C005 indicate three rice varieties that originated in china, respectively. e Comparison of the content of apigenin 5-O-glucoside in different rice varieties. The data are presented as mean ± SD, n = 3. g Enzymatic activity of OsUGT706D1 from two alleles. Allele I indicates the high producer genotype whereas the allele II indicates the low producer genotype. Assay was repeated three times and bars indicate mean ± SD, n = 3. **P < 0.01, Student’s t tests. h Kinetic parameters of OsUGT706D1 from two alleles. The data are presented as mean ± SD from two replicate independent assays. i Alignment of full amino acid sequences of OsUGT706D1 from two alleles. Black block indicates the conserved PSPG box domain. Nip nipponbare, ND not detected
