Fig.3: Ezh2 orchestrates the expression of genes critical for effector differentiation and memory formation | Nature Communications

Fig.3: Ezh2 orchestrates the expression of genes critical for effector differentiation and memory formation

From: Ezh2 phosphorylation state determines its capacity to maintain CD8+ T memory precursors for antitumor immunity

Fig.3

. a–c WT and Ezh2 −/− Pmel-1 cells were cultured in the presence of anti-CD3/CD28 Abs and IL-2 for 3 days. Cells were collected to extract RNA for sequencing. Using one-way ANOVA analysis, we selected transcripts with p < 0.01 and q < 0.01 for comparing paired groups and at least a 1.5-fold difference from the means for the paired groups. a Venn diagram shows the number of differentially expressed genes. b Table shows the signaling pathways most regulated by Ezh2 in activated Pmel-1 cells, which was identified by INGENUITY pathway analysis. c Heat map shows the cluster of genes associated with Cell Function and Maintenance in activated WT and Ezh2 −/− Pmel-1 cells. d, e Gene expression was assessed by real-time RT-PCR. *p < 0.05, and ***p < 0.001 (two-tailed unpaired t test). The data are representative of four independent experiments (d, e; mean ± SD)

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