Fig. 6: Inhibiting Akt-mediated phosphorylation of Ezh2 enhances the generation of TCMP. Ezh2 −/− Pmel-1 cells (Thy1.1+) were stimulated with anti-CD3/CD28 Ab + IL-2 for 36 h, followed by infection with MigR1 retrovirus encoding GFP, Ezh2, Ezh2S21D and Ezh2S21A, respectively | Nature Communications

Fig. 6: Inhibiting Akt-mediated phosphorylation of Ezh2 enhances the generation of TCMP. Ezh2 −/− Pmel-1 cells (Thy1.1+) were stimulated with anti-CD3/CD28 Ab + IL-2 for 36 h, followed by infection with MigR1 retrovirus encoding GFP, Ezh2, Ezh2S21D and Ezh2S21A, respectively

From: Ezh2 phosphorylation state determines its capacity to maintain CD8+ T memory precursors for antitumor immunity

Fig. 6

. At 7 days, after stimulation, these infected T cells were collected. a Experiment scheme. b Immunoblot analysis of Pmel-1 cells transduced with GFP, Ezh2, Ezh2S21D and Ezh2S21A, probed with Flag, H3K27me3 and H3. c RT-PCR analysis of gene expression in Pmel-1 cells transduced by indicated genes. d, e These 7 day-Pmel-1 cells (Thy1.1+) were transferred into sublethally irradiated B6 mice (Thy1.2+), followed by treatment with IL-2 and gp100-DCs for 3d after transfer. d Donor T cells were collected from the spleen, PB and LN at 6d after adoptive transfer, and measured by flow cytometric analysis. e Plots and graphs show the percentage of of Id3hi, KLRG1hi, CD62L+ and IFN-γ+ within donor T cells from the spleen. *p < 0.05, **p < 0.01, and ***p < 0.001 (two-tailed unpaired t test). The data are representatives of three experiments (b, c; mean ± SD) or two experiments with n = 4 mice per group in each (d, e; mean ± SD)

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