Fig. 2
From: RETRACTED ARTICLE: REST regulates the cell cycle for cardiac development and regeneration
Rest is required for neonatal cardiomyocyte proliferation and cardiac function. a A graph of experimental design for generating myocardial Rest knockout (RestimKO) at different postnatal (P) stages using the inducible TnTMerCreMer deleter mice. b Western blots showing effective deletion of Rest. c Survival curve of control and RestimKO mice, p < 0.001, log-rank (Mantel–Cox) testing between mice with Rest deletion at different stages and control (Tam-treated Rest+/+;TnTMerCreMer/+ mice). d Representative H&E staining and heart/body weight ratio revealing underdeveloped RestimKO hearts at P3 after Rest deletion at P1. n = 5/group. Scale bar = 100 µm. e–g Echocardiography showing structure defects and decreased left ventricle function of P3 RestimKO. n = 6 for RestimKO; n = 8 for Control. h–j FACS shows reduced percentage of cardiomyocytes isolated from P3 RestimKO hearts (h) and arrested cell cycle of the RestimKO cardiomyocytes at G0/G1 phase (i, j). k Immunostaining for cell cycle markers indicating reduced proliferation of Rest null cardiomyocytes. n = 4/group for FACS and immunostaining. Scale bar = 40 µm. Mean ± s.d., *p < 0.05, unpaired two-tailed Student’s t test
