Fig. 4
From: RETRACTED ARTICLE: REST regulates the cell cycle for cardiac development and regeneration
p21 deletion rescues embryonic heart defects resulting from myocardial Rest deletion. a Survival curve, no difference between control and p21 null (p21KO) or p21/Rest double knockout (DKO) group by log-rank (Mantel–Cox) test. p < 0.001 between RestmKO and each of other groups. b, c H&E-stained E12.5 heart sections showing that thin ventricular walls of Rest deleted hearts are restored by p21 deletion (n = 3/group). Scale bar = 100 μm. d Representative western blot revealing that upregulation of p21 and downregulation of Cyclin B, Cyclin E, and CDK1/2 in E12.5 Rest null hearts are restored to the normal levels of control hearts by p21 deletion. e Immunostaining for cell cycle markers indicating that p21 inactivation rescues the cell cycle defect resulting from Rest deletion (n = 4/group). Scale bar = 40 μm. f–i H&E staining (f scale bar = 100 μm), ratio of heart/body weight (g), and echocardiography (h, i) showing that P28 Rest/p21 DKO mice have cardiac structure and function comparable to control littermates (n = 6–8/group). Mean ± s.d. *p < 0.05 by one-way ANOVA followed by Tukey’s test. NS no significance
