Fig. 5
From: RETRACTED ARTICLE: REST regulates the cell cycle for cardiac development and regeneration
p21 deletion rescues neonatal heart defects resulting from Rest deletion. a Survival curve, no difference between control and p21 null (p21KO) or p21/Rest double knockout (DKO) group by log-rank (Mantel–Cox) test. p < 0.001 between RestimKO and each of other groups. b, c H&E-stained P3 heart sections showing that underdeveloped Rest null hearts are restored by p21 deletion (n = 5/group). Scale bar = 100 μm. d, e Echocardiograph showing p21 knockout rescues cardiac function resulting from Rest deletion at P1 (n = 6–8/group). f Representative western blot revealing upregulation of p21 and downregulation of Cyclin B, Cyclin E, and CDK1/2 in P3 Rest null hearts are restored by p21 deletion to the normal levels of control hearts. g Immunostaining for cell cycle markers indicating p21 inactivation rescues the cell cycle defect resulting from Rest deletion (n = 4/group). Scale bar = 40 μm. Controls were the Tam-treated Rest+/+;TnTMerCreMer/+ mice. Mean ± s.d. *p < 0.05 by one-way ANOVA followed by Tukey’s test
