Fig. 8
From: RETRACTED ARTICLE: REST regulates the cell cycle for cardiac development and regeneration
REST-dependent p21 expression is required for cardiac regeneration. a Schematic diagram showing experimental procedure of cardiac apex resection (AR) on neonatal mice. b, c Echocardiography indicates that mice with control (n = 9), single p21 knockout (p21KO, n = 8), and double knockout of Rest and p21 (DKO, n = 8) injured hearts have cardiac function comparable to that of the sham-operated control (s-Control, n = 9) at P28. Injured Rest single knockout (RestimKO, n = 9) hearts have significantly decreased left ventricular function comparable to injured control. d, e Quantitative Masson’s trichrome staining (n = 8/group) (d Scale bar = 100 µm) and EdU assays (n = 4/group) (e Scale bar = 40 µm) showing p21 inactivation reduces the cardiac fibrosis and rescues cardiomyocyte proliferation defect in the injured apex resulting from Rest deletion. Mean ± s.d. *p < 0.05 by one-way ANOVA followed by Bonferroni test. NS no significance
