Table 1 Amino acid-type selectively 13C-labeled OmpG samples produced for sequence-specific assignments and distance measurements

From: Structure of outer membrane protein G in lipid bilayers

Residue specific

[2-13C]- or [1,3-13C]-glycerol

GAFα,βYα,β (S)

2- and 1,3-uniform

GAVLS(Wα,β,γ)

2- and 1,3-TEMPQANDSG

RIGA(S)

2-SHLYGWAFV(QENDT)

GANDSH(LV)

1,3-MKINDT

GENDQPASR

 

GAFα,βYα,β SHVL

 
  1. Amino acids in brackets were accidentally labeled to a lower degree due to active biochemical pathways. Samples in the left column were prepared by adding 13C, 15N-labeled amino acids (or as specified) to 15NH4Cl-containing growth medium so all others appeared 15N- but not 13C-labeled. Samples in the right column were prepared by a “reverse” labeling scheme in which either [2-13C]- or [1,3-13C]-glycerol medium was used to produce the respective 13C-labeling pattern for the indicated amino acids, whereas all other amino acids were added in 15N-labeled form to the growth medium
Back to article page