Fig. 2
DCs interact with antigen-bearing LECs. a schematic of experimental design and antigen-bearing LEC, where LEC is red and antigen is green. Scale bar on image is 10 μm. b Still images from movie demonstrating prolonged association of DC-blue with white dashed outline, with antigen-green with white triangle and LEC-red. Scale bar on images is 15 μm. See Supplemental Movies for other examples. c Graph demonstrating the percentage of DCs within 2 μm of total LECs or only LECs that have antigen. Each dot represents the average from an individual lymph node. d Quantitation of DC–LEC cumulative interaction times from c. Analysis evaluated the duration that each DC interacted with each LECs that did or did not have antigen. e Quantitation of DC–LEC average interaction time. Analysis evaluated the average length of each interaction between DC and LEC. Seven-time lapse movies from four lymph nodes were imaged and quantified using IMARIS and Matlab software. f ICAM1 and CCL20 expression by antigen+ LECs compared to antigen—LECs 2 weeks after immunization. g Expression of C-C chemokine receptor (CCR6) and lymphocyte function-associated antigen–1 (LFA-1) on bone marrow-derived DCs grown for 6 days in GM-CSF. Statistical analysis was done using an unpaired t-test with a p-value of <0.0001. Error bars represent mean ± standard error of the mean
