Fig. 6

BatF3 DCs are necessary and sufficient for viral-archived antigen presentation. a Schematic of experimental design. Wild-type (WT) or K^bm8 mice were lethally irradiated and reconstituted with the indicated mixture of bone marrow. Following reconstitution, mice were infected with vaccinia expressing ovalbumin. Three weeks after immunization, violet proliferation dye (VPD)-labeled OT1 T cells were transferred into mice and T-cell division was assessed 3 days later. b Number of BatF3-dependent DCs was quantified using CD8 as a marker of BatF3 DCs in each strain used (Supplementary Fig. 3). Statistical analysis between groups was performed using an unpaired t-test, where BatF3: K^bm8 compared to BatF3 p-value was 0.0133, BatF3: K^bm8 compared to BatF3:WT was 0.0341, Batf3 compared to BatF3:WT was 0.0029, WT compared to BatF3 was 0.0059, and BatF3:WT compared to WT was 0.237. Shown are mean ± standard error of the mean. c Histograms of OT1 divided violet proliferation dye-labeled T cells from individual mice used in the chimera experiment. d Percent divided was calculated for all groups and statistical analysis between groups was performed using an unpaired t-test, where BatF3: K^bm8 compared to BatF3 p-value was 0.6125, BatF3: K^bm8 compared to BatF3:WT was <0.0001, Batf3 compared to BatF3:WT was <0.0001, and BatF3:WT compared to WT was 0.071. Shown are mean ± standard error of the mean. Six mice per group were evaluated from two separate lethally irradiated groups