Fig. 3
From: Voltage-gated sodium channels assemble and gate as dimers
14-3-3 inhibition abolishes DN effect. a Normalized current density at −20 mV recorded from transfected HEK293 cells. HEK293 cells were transfected with the different conditions illustrated at the bottom of the graph. WT corresponds to the wild-type cardiac sodium channel, L325R is the DN mutant, difopein (difo) corresponds to a 14-3-3 inhibitor and S460A is a mutant of the sodium channel where the 14-3-3 relevant phosphorylation site (S460) is mutated to alanine. Right panel displays representative family current traces for each conditions. b Normalized current density at −20 mV recorded from iCells transfected with different DN-mutants, GFP or WT. Transfection conditions are illustrated at the bottom of the graph. Right panel displays GFP normalized representative family current traces. n are noted on the bars, data are presented as mean ± SEM, *p < 0.05, ***p < 0.001, NS not significant
