Fig. 1

lar7Δ impairs telomerase activity. a–c Telomere southern blots of genomic DNA digested with EcoRI and hybridised with a telomeric probe. a Cells heterozygous for lar7∆ retained telomeres of wild-type length. Haploid lar7Δ cells were passaged every other day post sporulation and genomic DNA collected at the days stated. b The trt1 and rad51 genes were deleted in wild-type and lar7∆ strains and passaged for a week. Genomic DNA was collected and subjected to telomere southern blot. c The lar7 gene was deleted in the taz1∆, rap1∆ and rif1∆ strains and cells were passaged for a week. Genomic DNA was collected and subjected to telomere southern blot