Fig. 5
CPH1 is a central hub interacting with multiple proteins at the conoid. a Replicate mass spectrometry data sets generated with BirA fusion lines were analyzed using SFINX (http://sfinx.ugent.be/) to generate a proximity-based protein interaction network. Interactors of CPH1 and MyoH completely overlapped, and were partially shared with those for RNG2. Baits (BirA fusions)—deep blue nodes; prey (interactors)—light blue nodes. See Supplementary Data 2. b Localizations for the CPH1-MyoH-RNG2 interactome were analyzed for annotated and hypothetical proteins identified in the CPH1-MyoH-RNG2 interactome. Pie chart shows percentages of each category. The number in each category indicates protein number. N = 24 interactors. c Domains were analyzed for apical proteins and microtubule in the CPH1-MyoH-RNG2 interactome with InterPro. N = 17 interactors. b, c Protein localizations and domains for proteins in the CPH1-MyoH-RNG2 interactome were determined as defined in Supplementary Data 2. See also Supplementary Data 4, 5 and Supplementary Fig. 5 for GO term analysis. CC coiled coil, DCX double cortin microtubule-binding domain, myosin myosin A and H, prichextensin proline-rich motif present in plant cell wall, IDRs intrinsically disordered regions
