Fig. 6
CPH1 depletion has a minimal effect on protein stability of known conoid proteins. a Domain arrangements of RNG2, DCX, and MyoH as determined by InterPro. CRISPR fitness scores were derived from ref. 32. b Intracellular parasites grown in ±IAA for 24 h were stained with mouse anti-Ty (green) to detect the tagged proteins and rabbit anti-GAP45 (red) to outline the parasite followed by secondary antibodies conjugated to Alexa Fluor dyes. Scale = 2 μm. c Extracellular parasites collected after natural or mechanical egress from cultures grown in ±IAA for 40–44 h were stained with mouse anti-Ty (green) and rabbit anti-MLC1 (red) followed by secondary antibodies conjugated to Alexa Fluor dyes. Red arrow indicates position of the conoid. Scale = 2 μm. d Extracellular parasites were treated with 3 μM A23187 to protrude the conoid, and stained with rabbit anti-HA and mouse anti-Ty antibodies, and followed with reagents for proximity ligation assay (PLA). S6SL is a pre-conoid protein. Phase, DAPI (blue) and PLA (red) are shown. Scale = 2 μm. b–d Representative of three or more experiments with similar outcomes
